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Imaging of Pre-mRNA Splicing in Living Subjects Using A Genetically Encoded Luciferase Reporter
Mar 19, 2018Author:
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Title: Imaging of Pre-mRNA Splicing in Living Subjects Using A Genetically Encoded Luciferase Reporter

 Authors: Shi, YR; Liu, W; Zheng, HF; Li, ZQ; Shi, XR; Jiao, ZQ; Mao, WJ; Xie, JR; Tian, J; Wang, F

 Author Full Names: Shi, Yaru; Liu, Wei; Zheng, Haifeng; Li, Zhiqiang; Shi, Xiaorui; Jiao, Zhiqiang; Mao, Wenjie; Xie, Jinrong; Tian, Jie; Wang, Fu

 Source: BIOMEDICAL OPTICS EXPRESS, 9 (2):518-528; 10.1364/BOE.9.000518 FEB 1 2018

 Language: English

 Abstract: Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

 ISSN: 2156-7085

 IDS Number: FX2VZ

 Unique ID: WOS:000425923300013

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